Transcriptomic Analysis of Human Retinal Surgical Specimens Using jouRNAlMarie-Noëlle Delyfer 1,2,3,4, Najate Aït-Ali 1,2,3, Hawa Camara 1,2,3, Emmanuelle Clérin 1,2,3, Jean-François Korobelnik 4, José-Alain Sahel 1,2,3, Thierry Léveillard 1,2,3
1U968, Institut National de la Santé et de la Recherche Médicale, 2UMR S 968, Université Pierre et Marie Curie, 3UMR 7210, Centre National de la Recherche Scientifique, 4Départment d'Ophtalmologie, Centre Hospitalier Universitaire de Bordeaux
We used retinal samples from retinectomy for a transcriptomic analysis of retinal detachment. We developed a procedure that allows RNA conservation between the surgical blocks and the laboratory. We standardized a protocol to purify RNA by cesium chloride ultracentrifugation to assure that the purified RNAs are suitable for microarray analysis.
