Generation of Induced Pluripotent Stem Cells from Turner Syndrome (45XO) Fetal Cells for Downstream Modelling of Neurological Deficits Associated with the SyndromeNivedha Veerasubramanian *1, Vaishnavi Karthikeyan *1, Sridevi Hegde 2, Anandh Dhanushkodi 1, Shagufta Parveen 1
1Manipal Institute of Regenerative Medicine, Manipal Academy of Higher Education, Manipal, Karnataka, India-576104, 2Department of Medical Genetics, Manipal Hospitals
This protocol describes the generation of integration free iPSCs from fetal tissue fibroblasts through delivery of episomal plasmids by nucleofection followed by description of methods used for iPSC characterization and neuronal differentiation.
