Chicken Recombinant Limbs Assay to Understand Morphogenesis, Patterning, and Early Steps in Cell DifferentiationJessica Cristina Marín-Llera 1, Montse Fernández-Calderón 2, Jesús Chimal-Monroy 1
1Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 2Departamento de Anatomía y Biología Celular and IDIVAL, Facultad de Medicina, Universidad de Cantabria
Recombinant limbs are a powerful experimental model that allows for studying the process of cell differentiation and the generation of patterns under the influence of embryonic signals. This protocol presents a detailed method for generating recombinant limbs with chicken limb-mesodermal cells, adaptable to other cell types obtained from different organisms.
