S'identifier

INRS-Institut Armand-Frappier

3 ARTICLES PUBLISHED IN JoVE

Biology

Titration of Human Coronaviruses Using an Immunoperoxidase Assay

Francine Lambert¹, Helene Jacomy¹, Gabriel Marceau¹, Pierre J. Talbot¹

¹Laboratory of Neuroimmunovirology, INRS-Institut Armand-Frappier

In this video, we demonstrate an alternative method for detection and titering of viruses using an enzymatic antigen detection technique known as an immunoperoxidase assay. Here, we will show you how to collect your viral samples, prepare the cells for testing, and finally the immunoperoxidase assay using serial dilutions to determine the viral titer.

Biology

Preparation, Imaging, and Quantification of Bacterial Surface Motility Assays

Nydia Morales-Soto^1,2, Morgen E. Anyan¹, Anne E. Mattingly¹, Chinedu S. Madukoma¹, Cameron W. Harvey³, Mark Alber³, Eric Déziel⁴, Daniel B. Kearns⁵, Joshua D. Shrout^1,2,6

¹Department of Civil and Environmental Engineering and Earth Sciences, University of Notre Dame, ²Eck Institute for Global Health, University of Notre Dame, ³Department of Applied and Computational Mathematics and Statistics, University of Notre Dame, ⁴INRS-Institut Armand-Frappier, ⁵Department of Biology, Indiana University, ⁶Department of Biological Sciences, University of Notre Dame

Swarming motility is influenced by physical and environmental factors. We describe a two-phase protocol and guidelines to circumvent the challenges commonly associated with swarm assay preparation and data collection. A macroscopic imaging technique is employed to obtain detailed information on swarm behavior that is not provided by current analysis techniques.

Developmental Biology

Human Primary Trophoblast Cell Culture Model to Study the Protective Effects of Melatonin Against Hypoxia/reoxygenation-induced Disruption

Lucas Sagrillo-Fagundes^*1, Hélène Clabault^*1, Laetitia Laurent^*1, Andrée-Anne Hudon-Thibeault¹, Eugênia Maria Assunção Salustiano¹, Marlène Fortier¹, Josianne Bienvenue-Pariseault¹, Philippe Wong Yen¹, J. Thomas Sanderson¹, Cathy Vaillancourt¹

¹INRS-Institut Armand-Frappier

This manuscript presents a unique in vitro model of immunopurified human villous cytotrophoblast cells cultured under hypoxia/reoxygenation. This model is suitable to study the protective effects of promising treatments, such as melatonin, on pregnancy complications associated with increased oxidative stress and altered placental function.