Monitoring Equilibrium Changes in RNA Structure by 'Peroxidative' and 'Oxidative' Hydroxyl Radical FootprintingRavichandra Bachu *1, Frances-Camille S. Padlan *2, Sara Rouhanifard 2, Michael Brenowitz 2, Jörg C. Schlatterer 2
1Department of Chemistry, Hunter College , 2Department of Biochemistry, Albert Einstein College of Medicine
This protocol describes how to quantify the Mg(II)-dependent formation of RNA tertiary structure by two methods of hydroxyl radical footprinting.
