Genetic Manipulation of the Mouse Developing Hypothalamus through In utero ElectroporationRoberta Haddad-Tóvolli *1, Nora-Emöke Szabó *2, Xunlei Zhou 1, Gonzalo Alvarez-Bolado 1
1Institute of Anatomy and Cell Biology, University of Heidelberg , 2Institut de recherches cliniques de Montreal
Despite the functional and medical importance of the hypothalamus, in utero genetic manipulation of its development has rarely been attempted. We show a detailed procedure for in utero electroporation into the mouse hypothalamus and show representative results of total and partial (regional) hypothalamic transfection.
