S'identifier

Université Claude Bernard Lyon

1 ARTICLES PUBLISHED IN JoVE

Biology

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Marie-Christine Mariol^1,2, Ludivine Walter^1,2, Stéphanie Bellemin^1,2, Kathrin Gieseler^1,2

¹Université Claude Bernard Lyon, ²Centre de Génétique et de Physiologie Moléculaires et Cellulaires, CNRS UMR 5534

This protocol describes a rapid and low material consuming procedure for the integration of transgenic extrachromosomal arrays into the Caenorhabditis elegans genome using ultra violet (UV) irradiation. Furthermore, this protocol is particularly well suited for transgenic lines that transmit extrachromosomal arrays at a high rate.