S'identifier

Basque Foundation for Science

2 ARTICLES PUBLISHED IN JoVE

Biology

Organelle Transport in Cultured Drosophila Cells: S2 Cell Line and Primary Neurons.

Wen Lu^*1, Urko del Castillo^*1,2, Vladimir I. Gelfand¹

¹Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, ²IKERBASQUE, Basque Foundation for Science

Drosophila S2 cells and cultured neurons are great systems for imaging of motor-driven organelle transport in vivo. Here we describe detailed protocols for culturing both cell types, their imaging and analysis of transport.

Biochemistry

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Rory Nolan¹, Luis A. Alvarez¹, Samuel C. Griffiths², Jonathan Elegheert², Christian Siebold², Sergi Padilla-Parra^1,2,3,4

¹Cellular Imaging Group, Wellcome Centre Human Genetics, University of Oxford, ²Division of Structural Biology, Wellcome Centre Human Genetics, University of Oxford, ³Dynamic Structural Virology Group, Biocruces Health Research Centre, ⁴IKERBASQUE, Basque Foundation for Science

This protocol describes a calibration-free approach for quantifying protein homo-oligomerization in vitro based on fluorescence fluctuation spectroscopy using commercial light scanning microscopy. The correct acquisition settings and analysis methods are shown.