One Mouse, Two Cultures: Isolation and Culture of Adult Neural Stem Cells from the Two Neurogenic Zones of Individual MiceTara L. Walker 1, Gerd Kempermann 1,2
1Center for Regenerative Therapies Dresden, Technische Universität Dresden, 2German Center for Neurodegenerative Diseases (DZNE) Dresden
Here we describe a detailed protocol for the simultaneous generation of neural precursor cell cultures, as either adherent monolayers or neurospheres, from the subventricular zone and dentate gyrus of individual adult mice.
