S'identifier

AstraZeneca Global Medicines Development

1 ARTICLES PUBLISHED IN JoVE

Immunology and Infection

A Method for Generating Pulmonary Neutrophilia Using Aerosolized Lipopolysaccharide

Abraham B. Roos¹, Tove Berg¹, Kerstin M. Ahlgren¹, Johan Grunewald¹, Magnus Nord^1,2

¹Department of Medicine, Solna and CMM, Respiratory Medicine Unit, Karolinska Institutet, ²Safety Science, Global Regulator Affairs & Patient Safety, AstraZeneca Global Medicines Development

We describe a method for inducing neutrophilic pulmonary inflammation by challenge to aerosolized lipopolysaccharide by nebulization, to model acute lung injury. In addition, basic surgical techniques for lung isolation, tracheal intubation and bronchoalveolar lavage are also described.