Isolation of Distinct Cell Populations from the Developing Cerebellum by MicrodissectionLarra W. Yuelling 1, Fang Du 1, Peng Li 1, Renata E. Muradimova 1, Zeng-jie Yang 1
1Cancer Biology Program, Fox Chase Cancer Center, Temple University Health System
Nestin-expressing progenitors are a newly identified population of neuronal progenitors in the developing cerebellum. Using the microdissection technique presented here in combination with fluorescent-activated cell sorting, this cell population can be purified with no contamination from other cerebellar regions and can be cultured for further studies.
