Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling CulturesJulie Lessard 1, Julie Anne Côté 1, Marc Lapointe 1, Mélissa Pelletier 2, Mélanie Nadeau 1, Simon Marceau 1, Laurent Biertho 1, André Tchernof 1,2,3
1IUCPQ Research Center, 2CHU de Québec Research Center, 3Laval University
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
