Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid TumorsAlan J. Fox 1, Matthew C. Hiemenz 1, David B. Lieberman 1, Shrey Sukhadia 1, Barnett Li 1, Joseph Grubb 1, Patrick Candrea 1, Karthik Ganapathy 1, Jianhua Zhao 1, David Roth 1, Evan Alley 2,3, Alison Loren 2,3, Jennifer J. D. Morrissette 1
1Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, 2Division of Hematology/Oncology, Department of Medicine, Perelman School of Medicine, University of Pennsylvania, 3Abramson Cancer Center
This manuscript describes clinical protocols for two next-generation sequencing panels. One panel interrogates hematologic malignancies while the other panel targets genes commonly mutated in solid tumors. Molecular classification of driver mutations in human malignancies offers valuable prognostic and predictive information.
