Measurement and Analysis of Extracellular Acid Production to Determine Glycolytic RateShona A. Mookerjee 1,2, Martin D. Brand 1,2
1Touro University California College of Pharmacy, 2Buck Institute for Research on Aging
Glycolysis is a defining metabolic marker in multiple biological systems. Monitoring glycolysis by measuring the extracellular flux of H+ is common, but requires correction to be quantitative and unambiguous. Here, we demonstrate how to gather and correct extracellular flux data to distinguish between respiratory and glycolytic sources of extracellular acidification.
