Peering at Brain Polysomes with Atomic Force MicroscopyLorenzo Lunelli 1, Paola Bernabò 2, Alice Bolner 2, Valentina Vaghi 1, Marta Marchioretto 2, Gabriella Viero 2
1Laboratory of Biomolecular Sequence and Structure Analysis for Health, Fondazione Bruno Kessler, 2Institute of Biophysics, CNR Unit at Trento
While ribosome structure has been extensively characterized, the organization of polysomes is still understudied. To overcome this lack of knowledge, we present here a detailed preparation protocol for accurate imaging of mammalian polysomes by atomic force microscopy (AFM) in air and liquid.
