Genome-wide Analysis of HDAC Inhibitor-mediated Modulation of microRNAs and mRNAs in B Cells Induced to Undergo Class-switch DNA Recombination and Plasma Cell DifferentiationHelia N. Sanchez 1, Tian Shen 1,2, Dawn Garcia 1,3, Zhao Lai 1,3, Paolo Casali 1, Hong Zan 1
1Department of Microbiology, Immunology and Molecular Genetics, University of Texas Long School of Medicine at San Antonio, 2Xiangya School of Medicine, Cental South University, 3Greehey Children’s Cancer Research Institute, University of Texas Long School of Medicine at San Antonio
Epigenetic factors can interact with genetic programs to modulate gene expression and regulate B cell function. By combining in vitro B-cell stimulation, qRT-PCR, and high-throughput microRNA-sequence and mRNA-sequence approaches, we can analyze the epigenetic modulation of miRNA and gene expression in B cells.
