S'identifier

Massachusetts Institute of Technology and Harvard Medical School

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

A Simple, Robust, and High Throughput Single Molecule Flow Stretching Assay Implementation for Studying Transport of Molecules Along DNA

Kan Xiong^1,2, Paul C. Blainey^1,2

¹Broad Institute, Massachusetts Institute of Technology and Harvard Medical School, ²Dept. of Biological Engineering, Massachusetts Institute of Technology

This protocol demonstrates a simple, robust and high throughput single molecule flow-stretching assay for studying one-dimensional (1D) diffusion of molecules along DNA.