Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito LarvaePei-Wen Liu 1, Jia-Bao Xu 1, Yun-Qiao Dong 2, Xiao-Guang Chen 1, Jin-Bao Gu 1
1Department of Pathogen Biology, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, 2Reproductive Medical Centre of Guangdong Women and Children's Hospital
We report using an artificial intronic small RNA expression strategy to develop a non-defective recombinant Aedes aegypti densovirus (AaeDV) in vivo delivery system. A detailed procedure for the construction, packaging, and quantitative analysis of the rAaeDV vectors as well as for larval infection is described.
