S'identifier

University Hospital of Heidelberg

2 ARTICLES PUBLISHED IN JoVE

Neuroscience

Analysis of Immune Cells in Single Sciatic Nerves and Dorsal Root Ganglion from a Single Mouse Using Flow Cytometry

Asa S. Hidmark¹, Peter P. Nawroth^1,2,3, Thomas Fleming^1,3

¹Department of Medicine I and Clinical Chemistry, University Hospital of Heidelberg, ²Institute for Diabetes and Cancer IDC Helmholtz Center Munich, Germany & Joint Heidelberg-IDC Translational Diabetes Program, ³German Center for Diabetes Research (DZD)

Quantitative analysis of cell content within the murine sciatic nerve is difficult due to the scarcity of the tissue. This protocol describes a method for tissue digestion and preparation that provides sufficient cells for flow cytometry analysis of immune cell populations from nerves of individual mice.

Biochemistry

Plate-based Large-scale Cultivation of Caenorhabditis elegans: Sample Preparation for the Study of Metabolic Alterations in Diabetes

Katharina Kohl¹, Thomas Fleming^2,3, Kübra Acunman¹, Hans-Peter Hammes^1,4, Michael Morcos^*1, Andrea Schlotterer^*1

¹5th Medical Department, Medical Faculty Mannheim, Heidelberg University, ²Department of Internal Medicine, Heidelberg University, ³German Center for Diabetes Research (DZD), ⁴European Center for Angioscience, Medical Faculty Mannheim, Heidelberg University

This protocol describes a method for the large-scale cultivation of Caenorhabditis elegans on solid media. As an alternative to liquid culture, this protocol allows obtaining parameters of different scales under plate-based cultivation. This increases the comparability of results by omitting the morphological and metabolic differences between liquid and solid media culture.