Lens-free Video Microscopy for the Dynamic and Quantitative Analysis of Adherent Cell CultureCedric Allier 1, Romaric Vincent 1, Fabrice Navarro 2, Mathilde Menneteau 2, Lamya Ghenim 3,4, Xavier Gidrol 3, Thomas Bordy 1, Lionel Hervé 1, Olivier Cioni 1, Sabine Bardin 5, Michel Bornens 5, Yves Usson 4,6, Sophie Morales 1
1CEA, LETI, DTBS, LISA, Université Grenoble Alpes, 2CEA, LETI, DTBS, LBAM, Université Grenoble Alpes, 3CEA, INSERM, BIG, Université Grenoble Alpes, 4CNRS, FR CNRS 3425, 5CNRS, UMR 144, Molecular Mechanisms of Intracellular Transport, PSL Research University, Institut Curie, 6TIMC-IMAG
Lens-free video microscopy enables us to monitor cell cultures directly inside the incubator. Here we describe the full protocol used to acquire and analyze a 2.7 day long acquisition of cultured HeLa cells, leading to a dataset of 2.2 x 106 measurements of individual cell morphology and 10584 cell cycle tracks.
