A Simple Cell-based Immunofluorescence Assay to Detect Autoantibody Against the N-Methyl-D-Aspartate (NMDA) Receptor in BloodChia-Hsiang Chen 1,2, Yu-Syuan Chang 1
1Department of Psychiatry, Chang Gung Memorial Hospital-Linkou, 2Department and Graduate Institute of Biomedical Sciences, Chang Gung University
We ectopically expressed NR1 subunit of NMDA receptor tagged with green fluorescent protein in human embryonic cells (HEK293) as antigen to detect autoantibodies against NMDA receptor in the blood of patients suspected with autoimmune encephalitis. This simple method may be suitable for screening purposes in clinical settings.
