Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular NetworksGeorge Merz 1, Valerie Schwenk 1, Ruchit Shah 2, Carolyn Salafia 1,2, Phillip Necaise 2, Michael Joyce 1, Tom Villani 3, Michael Johnson 3, Nick Crider 3
1The Institute for Basic Research, The New York State Office for People with Developmental Disabilities, 2Placental Analytics LLC, 3Visikol Inc.
This study presents a protocol for the reversible tissue clearing, immunostaining, 3D-rendering and analysis of vascular networks in human placenta villi samples on the order of 1 - 2 mm3.
