S'identifier

Heidelberg University Biochemistry Center (BZH)

1 ARTICLES PUBLISHED IN JoVE

Biochemistry

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Isabel M. Schopp^1,2, Julien Béthune^1,2

¹Cluster of excellence CellNetworks, Heidelberg University, ²Heidelberg University Biochemistry Center (BZH)

We provide a step-by-step protocol for split-BioID, a protein fragments-complementation assay based on the proximity-labeling technique BioID. Activated on the interaction of two given proteins, it allows the proteomics analysis of context-dependent protein complexes in their native cellular environment. The method is simple, cost-effective and only requires standard laboratory equipment.