S'identifier

University of Fukui Faculty of Medical Sciences

1 ARTICLES PUBLISHED IN JoVE

JoVE Journal

Lipid Bilayer Experiments with Contact Bubble Bilayers for Patch-Clampers

¹Department of Molecular Physiology and Biophysics, University of Fukui Faculty of Medical Sciences

Here, we present a protocol for the formation of lipid bilayers using a contact bubble bilayer method. A water bubble is blown into an organic solvent, whereby a monolayer is formed at the water-oil interface. Two pipettes are manipulated to dock the bubbles to form a bilayer.