Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux AnalyzerShizuko Tachibana 1, Chao Chen 1, Oliver R. Zhang 1, Sarah V. Schurr 1, Cameron Hill 1, Ruixia Li 1, Ana M. Manso 1, Jianlin Zhang 1, Aleksander Andreyev 2, Anne N. Murphy 2, Robert S. Ross 1,3, Yoshitake Cho 1
1Division of Cardiology, Department of Medicine, University of California San Diego, 2Department of Pharmacology, University of California San Diego, 3Cardiology Section, Department of Medicine, Veterans Administration Healthcare, San Diego
The goal of this protocol is to illustrate how to use mouse neonatal cardiomyocytes as a model system to examine how various factors can alter oxygen consumption in the heart.
