S'identifier

Human Technopole

1 ARTICLES PUBLISHED IN JoVE

Neuroscience

In Vivo Targeting of Neural Progenitor Cells in Ferret Neocortex by In Utero Electroporation

Nereo Kalebic^1,2, Barbara Langen^1,3, Jussi Helppi¹, Hiroshi Kawasaki⁴, Wieland B. Huttner¹

¹Max Planck Institute of Molecular Cell Biology and Genetics, ²Human Technopole, ³Landesdirektion Sachsen, ⁴Department of Medical Neuroscience, Graduate School of Medical Sciences, Kanazawa University

Presented here is a protocol to perform genetic manipulation in the embryonic ferret brain using in utero electroporation. This method allows for targeting of neural progenitor cells in the neocortex in vivo.