Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43Vanessa S. Terra 1, Charles D. Plumptre 2, Emma C. Wall 3, Jeremy S. Brown 2, Brendan W. Wren 1
1Faculty of Infections and Tropical Diseases, Department of Infection Biology, London School of Hygiene and Tropical Medicine, 2Division of Medicine, University College London, 3Division of Infection and Immunity, University College London UCL
Here, we describe a S. pneumoniae serotype 1 strain 519/43 that can be genetically modified by using its ability to naturally acquire DNA and a suicide-plasmid. As proof of principle, an isogenic mutant in the pneumolysin (ply) gene was made.
