MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive BacteriaNoémie Mercier 1, Karine Prévost 2, Eric Massé 2, Pascale Romby 1, Isabelle Caldelari 1, David Lalaouna 1
1Université de Strasbourg, CNRS, 2Department of Biochemistry, Université de Sherbrooke
MAPS technology has been developed to scrutinize the targetome of a specific regulatory RNA in vivo. The sRNA of interest is tagged with a MS2 aptamer enabling the co-purification of its RNA partners and their identification by RNA sequencing. This modified protocol is particularly suited for Gram-positive bacteria.
