S'identifier

The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University

4 ARTICLES PUBLISHED IN JoVE

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Chemistry

Determination of the Gas-phase Acidities of Oligopeptides
Jianhua Ren 1, Ashish Sawhney 1, Yuan Tian 1, Bhupinder Padda 1, Patrick Batoon 1
1Department of Chemistry, University of the Pacific

The determination of the gas-phase acidities of cysteine-containing oligopeptides is described. The experiments are performed using a triple quadrupole mass spectrometer. The relative acidities of the peptides are measured using collision-induced dissociation experiments, and the quantitative acidities are determined using the extended Cooks kinetic method.

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Genetics

Single Molecule Analysis of Laser Localized Psoralen Adducts
Jing Huang 1, Himabindu Gali 1, Julia Gichimu 1, Marina A. Bellani 1, Durga Pokharel 1, Manikandan Paramasivam 1, Michael M. Seidman 1
1Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health

Lasers are frequently used in studies of the cellular response to DNA damage. However, they generate lesions whose spacing, frequency, and collisions with replication forks are rarely characterized. Here, we describe an approach that enables the determination of these parameters with laser localized interstrand crosslinks.

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Cancer Research

A Time-lapse, Label-free, Quantitative Phase Imaging Study of Dormant and Active Human Cancer Cells
Jing Huang *1,2, Peng Guo *1,2, Marsha A. Moses 1,2
1Vascular Biology Program, Boston Children's Hospital, 2Department of Surgery, Harvard Medical School and Boston Children's Hospital

Dormant and active cancer cell phenotypes were characterized using quantitative phase imaging. Cell proliferation, migration, and morphology assays were integrated and analyzed in one simple method.

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Medicine

Organ Ischemia-Reperfusion Injury by Simulating Hemodynamic Changes in Rat Liver Transplant Model
Yuan Yuan 1, Meng-hua Chen 1, Jing Huang 2, Yuan Tian 2, Ke Qin 3, Zhang Yuan 1, Wen-yan Wang 1, Zhi-jiang Wu 1, Xin-yue Tian 1, Yubin Zhang 4
1Department of Intensive Care Unit, The Second Affiliated Hospital of Guangxi Medical University, 2Department of hepatobiliary surgery, The Affiliated Ningbo Medical Center Lihuili Hospital of Medical School of Ningbo University, 3Department of transplantation, The Second Affiliated Hospital of Guangxi Medical University, 4Digital Technology and Engineering college, Ningbo University of Finance & Economics

This paper provides a detailed description of how to build an animal model of the anhepatic phase (liver ischemia) in rats to facilitate basic research into ischemia-reperfusion injury after liver transplantation.

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