Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and SegmentationAndré Dias 1, Gabriel G. Martins 1,2, Alexandre Lopes 1,3, Moisés Mallo 1
1Instituto Gulbenkian de Ciência, 2Faculdade de Ciências da Universidade de Lisboa, 3NOVA School of Science and Technology
Here, we describe computational tools and methods that allow visualization and analysis of three and four-dimensional image data of mouse embryos in the context of axial elongation and segmentation, obtained by in toto optical projection tomography, and by live imaging and whole-mount immunofluorescence staining using multiphoton microscopy.
