Chromatin Extraction from Frozen Chimeric Liver Tissue for Chromatin Immunoprecipitation AnalysisAndrea Pirosu 1,3, Lena Allweiss 1, Maura Dandri 1,2
1Department of Internal Medicine, University Medical Center Hamburg-Eppendorf, 2German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems site, 3Research Department Virus Immunology, Leibniz Institute for Experimental Virology
This protocol focuses on chromatin preparation from snap frozen tissues and it is suitable for Crosslinking Chromatin Immunoprecipitation (X-ChIP) followed by either quantitative PCR analysis (X-ChIP-qPCR) or next generation sequencing approaches (X-ChIP-seq).
