Defining Gene Functions in Tumorigenesis by Ex vivo Ablation of Floxed Alleles in Malignant Peripheral Nerve Sheath Tumor CellsJody Fromm Longo 1, Stephanie N. Brosius 2,3, Steven L. Carroll 1
1Department of Pathology and Laboratory Medicine, Medical University of South Carolina, 2Department of Pediatrics and Neurology, Perelman School of Medicine of the University of Pennsylvania, 3Division of Child Neurology, Children's Hospital of Philadelphia
Here, we present a protocol for performing gene knockouts that are embryonic lethal in vivo in genetically engineered mouse model-derived tumors and then assessing the effect that the knockout has on tumor growth, proliferation, survival, migration, invasion, and the transcriptome in vitro and in vivo.
