Topical Application Bioassay to Quantify Insecticide Toxicity for Mosquitoes and Fruit FliesBrook M. Jensen 1, Rachel A. Althoff 1, Sarah E. Rydberg 1, Emma N. Royster 1, Alden Estep 2, Silvie Huijben 1
1Center for Evolution and Medicine, School of Life Sciences, Arizona State University, 2United States Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural and Veterinary Entomology
We describe the methodology and importance of the topical application bioassay to measure insecticide susceptibility in mosquitoes and fruit flies. The presented assay is high-throughput, utilizes insect mass-thus allowing for calculating a mass-relativized lethal dose instead of concentration-and likely has lower variability than other similar methods.
