Developing and Testing Methylated Nano-Structured Dipeptides that Inhibit Src Kinase Activity In Vitro for Anti-Cancer ApplicationsAlaa F. Nahhas 1, Alrayan F. Nahhas 2, Thomas J. Webster 3,4,5
1Biochemistry Department, College of Science, King Abdulaziz University, 2Independent Scholar, 3School of Health Sciences and Biomedical Engineering, Hebei University of Technology, 4School of Engineering, Saveetha University, 5Programa de Pós-graduação em Ciência e Engenharia dos Materiais, UFPI
Presented here is a protocol for designing and testing dipeptides that can inhibit Src kinase enzyme activity using acellular and cellular assays for anti-cancer applications. The peptides formulated here (W-RCH3, WCH3-RCH3, and W-R(CH3)2) inhibited Src kinase with IC50 values of 510 nM, 916 nM, and 1 µM, respectively.
