Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell MultiomicsMateusz Strzelecki 1,4, Kelvin Yin 1, Carlos Talavera-López 2,3, Celia P. Martinez-Jimenez 1,4
1Helmholtz Pioneer Campus (HPC), Helmholtz Munich, 2Institute of Computational Biology, Computational Health Department, Helmholtz Munich, 3Division of Infectious Diseases and Tropical Medicine, Ludwig-Maximillian-Universität Klinikum, 4TUM School of Medicine, Technical University of Munich
Here, we present a protocol for isolating nuclei from flash-frozen, archived liver tissues for single-nucleus RNA-seq, ATAC-seq, and joint multiomics (RNA-seq and ATAC-seq).
