S'identifier

Yonsei University, Mirae Campus

3 ARTICLES PUBLISHED IN JoVE

Neuroscience

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

Chao He¹, Jin I. Lee², Noelle L'Etoile³, Damien O'Halloran¹

¹Department of Biological Sciences and Institute for Neuroscience, George Washington University, ²Fred Hutchinson Cancer Research Center, ³Department of Cell and Tissue Biology, University of California San Francisco

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

Biology

Cultivation of Caenorhabditis elegans in Three Dimensions in the Laboratory

Tong Y. Lee¹, Kyoung-hye Yoon¹, Jin I. Lee¹

¹Division of Biological Science and Technology, Yonsei University

We present a simple method to construct 3D nematode cultivation systems called NGT-3D and NGB-3D. These can be used to study nematode fitness and behaviors in habitats that are more similar to natural Caenorhabditis elegans habitats than the standard 2D laboratory C. elegans culture plates.

Behavior

A Smartphone-Based Imaging Method for C. elegans Lawn Avoidance Assay

Saebom Kwon¹, Jin I. Lee², Kyoung-hye Yoon¹

¹Department of Physiology, Mitohormesis Research Center, Yonsei University Wonju College of Medicine, ²Division of Biological Science and Technology, College of Science and Technology, Yonsei University, Mirae Campus

This article describes a simple, low-cost method of recording the lawn avoidance behavior of Caenorhabditis elegans, using readily available items such as a smartphone and a light emitting diode (LED) light box. We also provide a Python script to process the video file into a format more amenable for counting.