biology

Biology

cancer research

Cancer Research

biological techniques

Biological Techniques

immunology

Immunology

neuroscience

Neuroscience

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jove encyclopedia of experiments

JoVE Encyclopedia of Experiments

Online video encyclopedias of advanced research experiments for scientists in academia and biotech

Research

breast cancer

This collection features biomedical research methods employed in research laboratories to advance breast cancer prevention, detection, and treatment.

Breast Cancer

lung cancer

This collection highlights some of the latest protocols in lung cancer research. The collection covers techniques relating to the generation of lung cancer animal models, cancer cell characterization, treatment and detection strategies, in vitro culture studies, assays and isolation techniques that facilitate lung cancer research.

Lung Cancer

leukemia

Leukemia reflects cancer primarily resulting from an elevated number of white blood cells in the body. This collection features a set of in vitro assay techniques to analyze leukemic cell growth and metabolism, cell isolation and culture methods, approaches to characterize and study cellular morphology, and genetic manipulation procedures.

Leukemia

pancreatic cancer

The collection features protocols demonstrating the generation of pancreatic animal models, genetic engineering of pancreatic cancer cells, methods for in vitro modeling of pancreatic cancer, cell isolation & characterization techniques, and tumor cell behavior and response studies.

Pancreatic Cancer

colorectal cancer

Colorectal cancer originates within the colon or rectal regions of organisms. This collection highlights some fundamental research techniques in colorectal cancer relating to screening tests, genetic analysis, studies on the gut microbiome, therapeutic interventions, animal model generation, assay techniques, and cell behavior & metastatic responses.

Colorectal Cancer

skin cancer

This collection focusses on research approaches relating to melanoma and other types of skin cancer. The protocols feature techniques relevant to developing in vitro and in vivo skin carcinogenesis and metastatic models, cell culturing, skin regeneration approaches, genetic manipulation and sensitivity studies using skin cells, and assay techniques.

Skin Cancer

gynecologic cancer

This collection features protocols relating to the prevention, detection, and treatment of cancer originating from cells or regions of the female reproductive system. The protocols are relevant for cancer-targeting and detection approaches, metabolic assessment of cancer cells, developing in vivo and metastatic animal models, in vitro cell culture and assay techniques, and transplantation experiments.

Gynecologic Cancer

prostate cancer

This collection features techniques employed in prostate cancer research relating to cancer cell/organoid culture, processing, and staining, cell and tissue sampling, organ extractions, in vivo & in vitro cancer modeling, and cancer proteomics studies.

Prostate Cancer

gastrointestinal cancer

Gastrointestinal cancers include various tumor types originating in the regions or organs associated with the gastrointestinal tract. The collection comprises research protocols on cell isolation, culturing, and adhesion studies, tissue staining and histological analysis, DNA methylation studies, tissue preservation methods, exosome analysis, and generation of animal models.

Gastrointestinal Cancer

head and neck cancer

Head and neck cancers constitute carcinomas originating in and around the mucosal surfaces of the head and neck regions. This collection features protocols relating to cancer modeling in vitro, in ovo, and in vivo, cell isolation and culture, fluorescent in situ hybridization-related techniques, generation of decellularized tissue matrices, and drug-resistance characterization assays.

Head and Neck Cancer

urinary tract cancer

This collection features protocols relating to cancers originating in the regions or organs of the urinary or renal system. The techniques include organs, tissue, and cell harvesting procedures, organotypic culturing approaches, in vivo cancer modeling studies, decellularized tissue matrix generation, stem cell-related and electrophysiology studies, genetic-engineering strategies, tissue staining and imaging techniques, and protein expression analysis.

Urinary Tract Cancer

cancers of the nervous system

This collection features protocols relating to cancers originating in the nervous system. The techniques include intra-brain injections, in-vivo and in-vitro tumor modeling, cell and organelle culturing, extracellular vesicle harvesting and purification methodologies, proteomic analysis, organ isolation and preservation methods, cell migration assays, tissue engineering methods, and novel gene delivery approaches.

Cancers of the Nervous System

in vitro studies

In vitro studies

in vivo studies

In vivo studies

ex vivo studies

Ex vivo studies

sds-page based extraction of extracellular vesicles associated proteins: a procedure to extract proteins from evs and prepare them for in-gel digestion

sds-page-based-extraction-extracellular-vesicles-associated-proteins

SDS-PAGE Based Extraction of Extracellular Vesicles Associated Proteins: A Procedure to Extract Proteins from EVs and Prepare Them for In-Gel Digestion

Source: Lemaire, Q. et al. Characterization of Immune Cell-derived Extracellular Vesicles and Studying Functional Impact on Cell Environment. J. Vis. Exp. ...

npc neurosphere assay: an in vitro assay to assess the migratory response of neural precursor cells from the neurosphere

npc-neurosphere-assay-an-vitro-assay-to-assess-migratory-response

NPC Neurosphere Assay: An In Vitro Assay to Assess the Migratory Response of Neural Precursor Cells from the Neurosphere

Source: Williams, M. et al. Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs).&#160;J. Vis. Exp. (2018)
This video ...

sec-based isolation of microglia-derived extracellular vesicles: a technique to isolate extracellular vesicles from microglia conditioned culture media

sec-based-isolation-microglia-derived-extracellular-vesicles

SEC-based Isolation of Microglia-derived Extracellular Vesicles: A Technique to Isolate Extracellular Vesicles from Microglia Conditioned Culture Media

cryopreserved tissue section fixation and staining: a procedure to process frozen brain tissue sections for laser microdissection

cryopreserved-tissue-section-fixation-staining-procedure-to-process

Cryopreserved Tissue Section Fixation and Staining: A Procedure to Process Frozen Brain Tissue Sections for Laser Microdissection

Source: Comba, A. et al. Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, ...

cryopreservation of murine brain by snap freezing: a technique to rapidly freeze whole mouse brain specimen for histological analysis

cryopreservation-murine-brain-snap-freezing-technique-to-rapidly

Cryopreservation of Murine Brain by Snap Freezing: A Technique to Rapidly Freeze Whole Mouse Brain Specimen for Histological Analysis

blood-brain barrier mimic permeability assay: an in vitro assay to assess the permeability of bbb mimic to tracer molecules

blood-brain-barrier-mimic-permeability-assay-an-vitro-assay-to-assess

Blood-Brain Barrier Mimic Permeability Assay: An In Vitro Assay to Assess the Permeability of BBB Mimic to Tracer Molecules

Source: Le Joncour, V. et al. Predicting In Vivo Payloads Delivery using a Blood-Brain Tumor-Barrier in a Dish. J. Vis. Exp. (2019)
This video describes a ...

frozen mouse brain tissue sectioning: a procedure to obtain thin frozen tissue sections from frozen murine brain tissue

frozen-mouse-brain-tissue-sectioning-procedure-to-obtain-thin-frozen

Frozen Mouse Brain Tissue Sectioning: A Procedure to Obtain Thin Frozen Tissue Sections from Frozen Murine Brain Tissue

extracellular vesicle extraction: a technique to isolate extracellular vesicles from whole tissue specimens using differential centrifugation

extracellular-vesicle-extraction-technique-to-isolate-extracellular

Extracellular Vesicle Extraction: A Technique to Isolate Extracellular Vesicles from Whole Tissue Specimens Using Differential Centrifugation

Source: Hurwitz, S. N. et al. Extraction of Extracellular Vesicles from Whole Tissue. J. Vis. Exp. (2019)
This video describes the technique of extracting ...

microglial phagocytosis assay: an in vitro assay to establish microglial phagocytosis model for neuroblastoma cells using ipsc macrophages

microglial-phagocytosis-assay-an-vitro-assay-to-establish-microglial

Microglial Phagocytosis Assay: An In Vitro Assay to Establish Microglial Phagocytosis Model for Neuroblastoma Cells Using iPSC Macrophages

Source: Hall-Roberts, H. et al. In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages. J. Vis. Exp. (2021)
...

immunoaffinity based extraction of ubiquitinylated peptides: a technique to selectively extract ubiquitin tagged peptides from purified peptide fractions

immunoaffinity-based-extraction-ubiquitinylated-peptides-technique-to

Immunoaffinity Based Extraction of Ubiquitinylated Peptides: A Technique to Selectively Extract Ubiquitin Tagged Peptides from Purified Peptide Fractions

Source: Bezstarosti, K. et al. Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry. J. Vis. Exp. (2020)
This video ...

endothelial cell culture from rat brain microvessels: a procedure to isolate and culture endothelial cells from microvessels of rat brain

endothelial-cell-culture-from-rat-brain-microvessels-procedure-to

Endothelial Cell Culture from Rat Brain Microvessels: A Procedure to Isolate and Culture Endothelial Cells from Microvessels of Rat Brain

Source: Molino, Y. et al. Setting-up an&#160;In Vitro&#160;Model of Rat Blood-Brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated ...

cellular bioluminescence based assay: a high throughput method for combinatorial drug screening

cellular-bioluminescence-based-assay-high-throughput-method-for

Cellular Bioluminescence Based Assay: A High Throughput Method for Combinatorial Drug Screening

Source: Hu, Z. et al. A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells.&#160;J. Vis. ...

microglia isolation by immunostaining coupled cell sorting: an immunostaining method to isolate microglia from zebrafish brain cells using fluorescent activated cell sorting

microglia-isolation-immunostaining-coupled-cell-sorting-an

Microglia Isolation by Immunostaining Coupled Cell Sorting: An Immunostaining Method to Isolate Microglia from Zebrafish Brain Cells Using Fluorescent Activated Cell Sorting

Source: Mazzolini, J. et al. Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains. J. Vis. Exp. (2018)
In this ...

luciferase-tagged glioma stem cell model: a lentivirus-mediated method to prepare luciferase-tagged glioma stem cells for in vitro therapeutic studies

luciferase-tagged-glioma-stem-cell-model-lentivirus-mediated-method

Luciferase-tagged Glioma Stem Cell Model: A Lentivirus-mediated Method to Prepare Luciferase-tagged Glioma Stem Cells for In Vitro Therapeutic Studies

Source: Hu, Z. et al. A Rapid Screening Workflow to Identify Potential Combination Therapy for GBM using Patient-Derived Glioma Stem Cells. J. Vis. Exp. ...

isolation of nuclei from fresh frozen glioma tissues: a method to obtain intact nuclei from glioma tumor samples

isolation-nuclei-from-fresh-frozen-glioma-tissues-method-to-obtain

Isolation of Nuclei from Fresh Frozen Glioma Tissues: A Method to Obtain Intact Nuclei from Glioma Tumor Samples

Source: Narayanan, A. et al. Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq. J. Vis. Exp. (2020)
This video ...

gradient centrifugation based purification of nuclei: a technique to obtain ultrapure fraction of intact nuclei using density gradient centrifugation

gradient-centrifugation-based-purification-nuclei-technique-to-obtain

Gradient Centrifugation Based Purification of Nuclei: A Technique to Obtain Ultrapure Fraction of Intact Nuclei Using Density Gradient Centrifugation

Source: Narayanan, A. et al. Nuclei Isolation from Fresh Frozen Brain Tumors for Single-Nucleus RNA-seq and ATAC-seq. J. Vis. Exp. (2020)
In this video, ...

fluorescence based transwell macrophage attraction assay: an in vitro method to study tumor-macrophage interaction through chemical-induced chemotaxis

fluorescence-based-transwell-macrophage-attraction-assay-an-vitro

Fluorescence Based Transwell Macrophage Attraction Assay: An In Vitro Method to Study Tumor-Macrophage Interaction Through Chemical-Induced Chemotaxis

Source: An, Z. et al. In Vitro Assay to Study Tumor-macrophage Interaction. J. Vis. Exp. (2019)
In this video, we study tumor-macrophage interaction ...

dna synthesis assay: a technique to assess dna synthesis in proliferating cells using radioactive tritiated thymidine incorporation

dna-synthesis-assay-technique-to-assess-dna-synthesis-proliferating

DNA Synthesis Assay: A Technique to Assess DNA Synthesis in Proliferating Cells Using Radioactive Tritiated Thymidine Incorporation

Source: Williams, M. et al. Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs). J. Vis. Exp. (2018)
In this video, we ...

purification of subpopulations of extracellular vesicles: a technique to purify subpopulations of extracellular vesicles using density gradient ultracentrifugation

purification-subpopulations-extracellular-vesicles-technique-to

Purification of Subpopulations of Extracellular Vesicles: A Technique to Purify Subpopulations of Extracellular Vesicles Using Density Gradient Ultracentrifugation

Source: Urwitz, S. N. et al. Extraction of Extracellular Vesicles from Whole Tissue. J. Vis. Exp. (2019)
This video demonstrates the density gradient ...

astrocyte isolation: a method to obtain pure preparation of mouse cortical astrocytes

astrocyte-isolation-method-to-obtain-pure-preparation-mouse-cortical

Astrocyte Isolation: A Method to Obtain Pure Preparation of Mouse Cortical Astrocytes

Source: Schildge, S. et al. Isolation and Culture of Mouse Cortical Astrocytes. J. Vis. Exp. (2013)
In this video, we describe the method to obtain a pure ...

blood-brain tumor barrier or bbtb model: an adaption of the blood-brain barrier or bbb model to evaluate delivery of therapeutics to the tumor

blood-brain-tumor-barrier-or-bbtb-model-an-adaption-blood-brain

Blood-Brain Tumor Barrier or BBTB Model: An Adaption of the Blood-Brain Barrier or BBB Model to Evaluate Delivery of Therapeutics to the Tumor

Source: Le Joncour, V. et al. Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish. J. Vis. Exp. (2019)
In this video, we ...

generation of induced neural stem cells (inscs) from peripheral blood mononuclear cells (pbmncs): a sendai virus-based procedure to reprogram pbmncs into inscs

generation-induced-neural-stem-cells-inscs-from-peripheral-blood

Generation of Induced Neural Stem Cells (iNSCs) from Peripheral Blood Mononuclear Cells (PBMNCs): A Sendai Virus-based Procedure to Reprogram PBMNCs into iNSCs

Source: Zheng, W. et al. Generation of Induced Neural Stem Cells from Peripheral Mononuclear Cells and Differentiation Toward Dopaminergic Neuron ...

repetitive tumor challenge assay: an in vitro culture assay to evaluate chimeric antigen receptor (car) t cells for repetitive tumor killing potential

repetitive-tumor-challenge-assay-an-vitro-culture-assay-to-evaluate

Repetitive Tumor Challenge Assay: An In Vitro Culture Assay to Evaluate Chimeric Antigen Receptor (CAR) T Cells for Repetitive Tumor Killing Potential

Source: Wang, D. et al. In Vitro Tumor Cell Rechallenge for Predictive Evaluation of Chimeric Antigen Receptor T cell Antitumor Function.&#160;J. Vis. ...

isolation of circulating cell-free dna (cfdna): a silica-based membrane procedure to extract cfdna from blood plasma of cancer patients

isolation-circulating-cell-free-dna-cfdna-silica-based-membrane

Isolation of Circulating Cell-free DNA (cfDNA): A Silica-based Membrane Procedure to Extract cfDNA from Blood Plasma of Cancer Patients

Source: Palande, V. et al. Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients. J. Vis. Exp. (2020)
This video describes a silica-based ...

automated capillary electrophoresis based fragment size distribution analysis of cfdna: a dna microchip-based method to study size distribution of dna fragments isolated from biofluids

automated-capillary-electrophoresis-based-fragment-size-distribution

Automated Capillary Electrophoresis Based Fragment Size Distribution Analysis of cfDNA: A DNA Microchip-based Method to Study Size Distribution of DNA Fragments Isolated from Biofluids

Source: Palande, V. et al. Detection of Cell-Free DNA in Blood Plasma Samples of Cancer Patients. J. Vis. Exp. (2020)
In this video, we analyze the size ...

droplet digital polymerase chain reaction: a method to generate nanodroplet pcr reactions for detecting rare tumor mutations

droplet-digital-polymerase-chain-reaction-method-to-generate

Droplet Digital Polymerase Chain Reaction: A Method to Generate Nanodroplet PCR Reactions for Detecting Rare Tumor Mutations

Source: Bonner, E. R. et al. Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids. J. Vis. Exp. (2019)
In this video, we ...

msc seeded scaffold preparation: a technique to prepare scaffold seeded with mesenchymal stem cells for surgical implantation into murine model

msc-seeded-scaffold-preparation-technique-to-prepare-scaffold-seeded

MSC Seeded Scaffold Preparation: A Technique to Prepare Scaffold Seeded with Mesenchymal Stem Cells for Surgical Implantation into Murine Model

Source: Sheets, K. T. et al. Image-Guided Resection of Glioblastoma and Intracranial Implantation of Therapeutic Stem Cell-seeded Scaffolds. J. Vis. Exp. ...

fluorescent labeling of glioma cells: a lentiviral vector-based transfection method to obtain glioma cells expressing fluorescent protein for deep tissue imaging

fluorescent-labeling-glioma-cells-lentiviral-vector-based

Fluorescent Labeling of Glioma Cells: A Lentiviral Vector-based Transfection Method to Obtain Glioma Cells Expressing Fluorescent Protein for Deep Tissue Imaging

Source: Benitez, J. A. et al. Fluorescence Molecular Tomography for In Vivo Imaging of Glioblastoma Xenografts. J. Vis. Exp. (2018)
In this video, we ...

brain tumor stem cell (btsc) migration assay for drug treatment studies: an in vitro live-cell imaging technique to analyze the effect of drug treatment on migration of btscs

brain-tumor-stem-cell-btsc-migration-assay-for-drug-treatment-studies

Brain Tumor Stem Cell (BTSC) Migration Assay for Drug Treatment Studies: An In Vitro Live-cell Imaging Technique to Analyze the Effect of Drug Treatment on Migration of BTSCs

Source: Restall, I. et al. Live-Cell Imaging Assays to Study Glioblastoma Brain Tumor Stem Cell Migration and Invasion. J. Vis. Exp. (2018)
In this video, ...

brain tumor stem cell (btsc) neurosphere invasion assay: an in vitro assay to analyze the effect of drug treatment on btsc invasion from neurosphere

brain-tumor-stem-cell-btsc-neurosphere-invasion-assay-an-vitro-assay

Brain Tumor Stem Cell (BTSC) Neurosphere Invasion Assay: An In Vitro Assay to Analyze the Effect of Drug Treatment on BTSC Invasion from Neurosphere

Source: Restall, I. et al. Live-Cell imaging assays to study glioblastoma brain tumor stem cell migration and invasion. J. Vis. Exp.&#160;(2018)
This ...

co-culturing glioblastoma cells on patterned neurons: a technique to monitor the migration of glioblastoma cells on rat hippocampal neurons in vitro

co-culturing-glioblastoma-cells-on-patterned-neurons-technique-to

Co-Culturing Glioblastoma Cells on Patterned Neurons: A Technique to Monitor the Migration of Glioblastoma Cells on Rat Hippocampal Neurons In Vitro

Source: Guyon, J. et al. Co-culture of Glioblastoma Stem-like Cells on Patterned Neurons to Study Migration and Cellular Interactions. J. Vis. Exp. 
...

isolation and culture of primary brain pericytes: a procedure to isolate and culture pericytes from bovine brain capillaries without endothelial cell contamination

isolation-culture-primary-brain-pericytes-procedure-to-isolate

Isolation and Culture of Primary Brain Pericytes: A Procedure to Isolate and Culture Pericytes from Bovine Brain Capillaries Without Endothelial Cell Contamination

Source: H&#248;rlyck, S. et al. Culture of Brain Capillary Pericytes for Cytosolic Calcium Measurements and Calcium Imaging Studies.&#160;J. Vis. Exp. ...

fluorescent peptide zymography: a modified technique to detect protease activity using fluorogenic substrates in zymogram gels

fluorescent-peptide-zymography-modified-technique-to-detect-protease

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels

Source: Deshmukh, A. A. et al. Detection of Protease Activity by Fluorescent Peptide Zymography. J. Vis. Exp. (2019)
In this video, we perform zymography, ...

generation of microtumors: an in vitro culture technique to culture 3d microtumors to analyze response to drug treatment

generation-microtumors-an-vitro-culture-technique-to-culture-3d

Generation of Microtumors: An In Vitro Culture Technique to Culture 3D Microtumors to Analyze Response to Drug Treatment

Source: Gilbert, A. N. et al. Generation of Microtumors Using 3D Human Biogel Culture System and Patient-derived Glioblastoma Cells for Kinomic Profiling ...

in vitro phagocytosis assay: a live imaging based method to study real-time astrocyte mediated synaptosome phagocytosis

in-vitro-phagocytosis-assay-live-imaging-based-method-to-study-real

In Vitro Phagocytosis Assay: A Live Imaging Based Method to Study Real-time Astrocyte Mediated Synaptosome Phagocytosis

Source: Byun, Y. G., Chung, W. S. A Novel In Vitro Live-imaging Assay of Astrocyte-mediated Phagocytosis Using pH Indicator-conjugated Synaptosomes. J. ...

ex vivo electroporation of chick embryo cerebellar slices: a method to introduce plasmid dna encoding green fluorescent protein to visualize granular cell development

ex-vivo-electroporation-chick-embryo-cerebellar-slices-method-to

Ex Vivo Electroporation of Chick Embryo Cerebellar Slices: A Method to Introduce Plasmid DNA Encoding Green Fluorescent Protein to Visualize Granular Cell Development

Source: Hanzel, M. et al.&#160;Ex Vivo&#160;Culture of Chick Cerebellar Slices and Spatially Targeted Electroporation of Granule Cell Precursors.&#160;J. ...

single cell electroporation in organotypic brain slice cultures: an in vitro technique to deliver plasmids inside targeted neurons in brain hippocampal slices

single-cell-electroporation-organotypic-brain-slice-cultures-an-vitro

Single Cell Electroporation in Organotypic Brain Slice Cultures: An In Vitro Technique to Deliver Plasmids Inside Targeted Neurons in Brain Hippocampal Slices

Source: Keener, D. G. et al. Single-Cell Electroporation Across Different Organotypic Slice Culture of Mouse Hippocampal Excitatory and Class-Specific ...

Encyclopedia Of Experiments - cancer research