S'identifier

Mitochondrial Isolation for Transmitochondrial Cybrid Generation

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02:46 min

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March 17th, 2023

DOI :

10.3791/200013-v

March 17th, 2023

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Ruth Soler-Agesta¹, Joaquín Marco-Brualla¹, Patricio Fernández-Silva¹^,², Pilar Mozas¹^,³, Alberto Anel¹, Raquel Moreno Loshuertos¹^,²

¹Department of Biochemistry, University of Zaragoza, ²Institute for Biocomputation and Physics of Complex Systems, University of Zaragoza, ³Sequencing and Functional Genomics Unit, University of Zaragoza-IACS

Transcription

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Keywords Mitochondrial Isolation

Transmitochondrial Cybrid Generation

Hypotonic Buffer

Mitochondrial Protein Quantification

Nuclear Contamination Assessment

Quantitative PCR

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