establishment of murine chronic root ganglion compression model

Protocole Tuina normalisé pour le modèle de compression Rat DRG

In clinical settings, it is common to observe neurological pathological pain caused by nerve root compression due to various reasons. The most typical form of this neuropathic pain is lumbar disc herniation (LDH), which is often persistent, recurrent, and difficult to cure. Approximately 9% of the global population is affected by LDH, leading to significant social and economic burdens1. The incidence of this type of neuropathic pain is increasing yearly, with a trend toward younger patients, due to changes in human production and lifestyle2. Despite the use of non-steroidal painkillers, patients&#39; symptoms cannot be completely alleviated. As a result, alternative therapies, such as Tuina, for treating pain caused by LDH have gained increasing attention.
Tuina therapy, a form of conservative treatment for LDH, is widely recommended in various clinical practice guidelines worldwide for preventing and treating lower back pain3,4. Research has shown that Tuina can significantly lower inflammatory factors such as serum IL-6 and tumor necrosis factor-alpha (TNF-&#945;) levels in LDH patients while improving patients&#39; pain and lumbar function impairment5. However, the specific mechanism behind Tuina therapy&#39;s pain-relieving effects remains unclear.
Animal models are a valuable tool for studying neuropathic pain caused by LDH6. They allow for behavioral measurements to evaluate the effectiveness of Tuina therapy and provide samples of the pathological physiology of LDH. For example, samples from the dorsal root ganglia in the thigh can be taken to verify changes in dorsal root ganglion cells. The chronic compression of the Dorsal Root Ganglion (CCD) model is commonly used to evaluate the pathological physiology of LDH, as it causes damage to the morphology of dorsal root ganglion cells that are consistent with the pathological changes seen in clinical cases of nerve compression caused by disc herniation7.
Many scholars have conducted several animal experiments on acupressure analgesia8,9,10. However, when implementing acupressure operations on animal models, they often imitate human acupressure. The therapeutic effect of acupressure is affected by factors such as the size, frequency, and the direction of the applied force11,12,13. If the experiment lacks a unified acupressure standard, such as the force, frequency, and duration of the operation, this may cause some deviation in the experimental results. This article introduces a set of acupressure treatment plans based on the characteristics of CCD rats, and promotes the development of standardized acupressure operations in animal models.

Pleins feux sur l’auteur : Effet analgésique de la tuina sur des modèles de rats avec compression de la douleur ganglionnaire de la racine dorsale  

Effet analgésique du Tuina sur les modèles de rats avec compression de la douleur ganglionnaire de la racine dorsale

Watch this Scientific Journal Video about Establishment of Murine Chronic Root Ganglion Compression Model at JoVE.com

Establishment of Murine Chronic Root Ganglion Compression Model  

Mise en place d’un modèle de compression chronique des ganglions racinaires murins

Pour établir le modèle animal, commencez par fixer un rat anesthésié sur une planche de mousse, en attachant ses membres et ses incisives à l’aide d’élastiques. Essuyez la zone préparée avec un tampon imbibé d’alcool à 75 %. Ensuite, utilisez des ciseaux pour faire une incision de deux à trois centimètres à travers la peau, le fascia superficiel et le fascia profond couche par couche.Localisez l’épine iliaque antéro-supérieure, suivie des troisième et quatrième apophyses épineuses. Serrez l’apophyse épineuse avec une pince dentée et soulevez-la pour permettre aux ciseaux d’être près du côté droit de l’apophyse épineuse. Coupez le muscle attaché au côté droit de l’apophyse épineuse.Ensuite, disséquez carrément le muscle attaché à la surface externe de la plaque vertébrale jusqu’à ce qu’une résistance se fasse sentir. De même, disséquez carrément le muscle et le fascia de l’articulation zygapophysaire. Utilisez une sonde en forme de L pour localiser le foramen intervertébral.Ensuite, insérez une tige en acier inoxydable en forme de L dans les quatrième et cinquième foramens intervertébraux lombaires. Procédez à la suture du muscle, du fascia et de la peau couche par couche à l’aide de sutures 3-0. Placez le rat dans une boîte thermostatique jusqu’à ce qu’il se réveille et observez la fonction de la patte arrière droite du rat pour assurer sa normalité.

establishment-of-murine-chronic-root-ganglion-compression-model

Research

JoVE Journal

Medicine

Establishment of Murine Chronic Root Ganglion Compression Model

150 vues. Pour établir le modèle animal, commencez par fixer un rat anesthésié sur une planche de mousse, en attachant ses membres et ses incisives à l’aide d’élastiques. Essuyez la zone préparée avec un tampon imbibé d’alcool à 75 %. Ensuite, utilisez des ciseaux pour faire une incision de deux à trois centimètres à travers la peau, le fascia superficiel et le fascia profond couche par couche.Localisez l’épine iliaque antéro-supérieure, suivie des troisième et quatrièm...

Vidéo: Establishment of Murine Chronic Root Ganglion Compression Model  

Analgesic Effect of Tuina on Rat Models with Compression of the Dorsal Root Ganglion Pain

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar disc herniation, spinal canal stenosis, and intervertebral foramen stenosis. Although Tuina, a traditional Chinese manual therapy, has shown analgesic effects in clinical practice for the treatment of neuropathic pain, its underlying neurobiological mechanisms remain unclear. Animal models are essential for elucidating the basic principles of Tuina. In this study, we propose a standardized Tuina protocol for rats with compression of the dorsal root ganglion (DRG), which involves inducing DRG compression by inserting a stainless steel rod into the intervertebral foramen, performing Tuina manipulation with specific parameters of location, intensity, and frequency in a controlled environment, and assessing the behavioral and histopathological outcomes of Tuina treatment. This article also discusses the potential clinical implications and limitations of the study and suggests directions for future research on Tuina.

This article presents a manipulation for treating chronic compression of the dorsal root ganglion in rats using Tuina therapy, along with a method for evaluating its effectiveness based on pain behavior and histopathological results.

Neuropathic pain is a prevalent condition that affects 6.9%-10% of the population and results from nerve damage due to various etiologies, such as lumbar ...