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Characterization of Multi-subunit Protein Complexes of Human MxA Using Non-denaturing Polyacrylamide Gel-electrophoresis

DOI :

10.3791/54683-v

October 28th, 2016

October 28th, 2016

9,901 Views

1Institute for Medical Virology, University of Zurich, 2Friedrich Miescher Institute for Biomedical Research

This article describes a simple and rapid protocol to evaluate the oligomeric state of the dynamin-like GTPase MxA protein from lysates of human cells using a combination of non-denaturing PAGE with western blot analysis.

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Keywords Multi subunit Protein Complexes

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