The overall goal of this procedure is to observe larval stage Drosophila crawling in a linear agarose channel to study the development of neural circuits. This method can help answer key questions in the fields of neuro ethology and developmental
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The Drosophila larva is a powerful model system to study neural control of behavior. This publication describes the use of linear agarose channels to elicit sustained bouts of linear crawling and methods to quantify the dynamics of larval structures during repetitive crawling behavior.