The overall goal of using a GFP-tagged construct is to confirm the identity of a previously unknown protein using the GFP as a handle. This method can help with the functional identification of orphan genes when a specific enzyme activity is not a
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A construct encoding TMEM184A with a GFP tag at the carboxy-terminus designed for eukaryotic expression, was employed in assays designed to confirm the identification of TMEM184A as a heparin receptor in vascular cells.