Diptheria Toxin (DPT) Myocarditis Induction and Light-sheet Microscope Sample Preparation
2:12
Light-sheet Microscopy and Image Post-processing
5:16
Results: Representative 3D Recordings of Whole Organ Light-sheet Microscopy
5:58
Conclusion
Transcription
The overall goal of this protocol is to chemically clear the heart of a mouse, with an induced cardiac arrhythmia for a whole organ visualization of the leukocytic cardiac infiltrate by Fluorescence Light Sheet Microscopy. This method can help ans
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Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.