JoVE Logo
Centre de ressources universitaires

S'identifier

University of Duisburg/Essen

2 ARTICLES PUBLISHED IN JoVE

image

Immunology and Infection

Direct Observation of Phagocytosis and NET-formation by Neutrophils in Infected Lungs using 2-photon Microscopy
Mike Hasenberg 1, Anja Köhler 1, Susanne Bonifatius 1, Andreas Jeron 2, Matthias Gunzer 1
1Institute for Molecular and Clinical Immunology, Otto-von-Guericke University Magdeburg, 2Department of Immunoregulation, Helmholtz Center for Infection Research

We show, how to use 2-photon microscopy for the observation of the dynamics of neutrophil granulocytes in infected lungs while they phagocytose pathogens or produce neutrophil extracellular traps (NETs).

image

Medicine

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy
Linda Männ 1, Anika Klingberg 2, Matthias Gunzer 2, Mike Hasenberg 3
1Department of Translational Skin Cancer Research, University of Duisburg/Essen, 2Institute for Experimental Immunology and Imaging, University of Duisburg/Essen, 3Imaging Center Essen, Electron Microscopy Unit, University Hospital of Essen

Here, we describe a light-sheet microscopy approach to visualize the cardiac CD45+ leukocyte infiltrate in a murine model of aseptic fulminant myocarditis, which is induced by the intratracheal diphtheria toxin treatment of CD11c.DTR mice.

JoVE Logo

Confidentialité

Conditions d'utilisation

Politiques

Recherche

Enseignement

À PROPOS DE JoVE

Copyright © 2024 MyJoVE Corporation. Tous droits réservés.