Name: march 2016 - this month in jove: rna interference in mosquitoes, ipscs derived from nasal epithelia, air sampling of atmospheric aerosols, and autologous micro-grafts for skin lesions
Uploaded: 2016-03-02T17:00:00
Duration: 3 min 41 s
Description: Watch this Scientific Journal Video about March 2016 - This Month in JoVE: RNA interference in Mosquitoes, iPSCs Derived from Nasal Epithelia, Air Sampling of Atmospheric Aerosols, and Autologous Micr

<h2 class="jove_title"><a href="https://www.jove.com/video/53579">Tissue Characterization after a New Disaggregation Method for Skin Micro-Grafts Generation</a></h2>Valeria Purpura1, Elena Bondioli1, Antonio Graziano2, Letizia Trovato2, Davide Melandri1, Martina Ghetti1, Andrea Marchesini3, Maria Gabriella Cusella De Angelis4,5, Laura Benedetti4,5, Gabriele Ceccarelli4,5, Michele Riccio31Burns Centre and Emilia Romagna Regional Skin Bank, 2Human Brain Wave srl, 3Plastic and Reconstructive Surgery, AOU &ldquo;Ospedali Riuniti&rdquo;, 4Department of Public Health, Experimental Medicine, Anatomy and Forensic, University of Pavia, 5C.H.T Centre for Health Technologies, University of PaviaThe protocol describes a new method to disaggregate human tissues and to create autologous micro-grafts that, combined with collagen sponges, give rise to human bio-complexes ready to use in the treatment of skin lesions. Further, this system preserves cell viability of micro-grafts at different times after mechanical disaggregation.<h2 class="jove_title"><a href="https://www.jove.com/video/53444">Air-sampled Filter Analysis for Endotoxins and DNA Content</a></h2>Naama Lang-Yona1,2, Yinon Mazar1, Michal Pardo1, Yinon Rudich11Department of Earth and Planetary Sciences, Weizmann Institute of Science, 2Multiphase Chemistry Department, Max Planck InstituteTwo complementary analyses of atmospheric biological particles from air sampled filters are described herein: the extraction and detection of endotoxin, and of DNA.<h2 class="jove_title"><a href="https://www.jove.com/video/53738">RNAi Trigger Delivery into Anopheles gambiae Pupae</a></h2>Kimberly Regna1, Rachel M. Harrison1, Shannon A. Heyse1, Thomas C. Chiles1, Kristin Michel2, Marc A. T. Muskavitch1,31Biology Department, Boston College, 2Division of Biology, Kansas State University, 3Discovery Research, BiogenRNA interference (RNAi) is an extremely valuable tool for uncovering gene function. However, the ability to target genes using RNAi during pre-adult stages is limited in the major human malaria vector Anopheles gambiae. We describe an RNAi protocol to reduce gene function via direct injection during pupal development.<h2 class="jove_title"><a href="https://www.jove.com/video/53814">Cultivate Primary Nasal Epithelial Cells from Children and Reprogram into Induced Pluripotent Stem Cells</a></h2>Ashley Ulm1, Christopher N. Mayhew2, Jason Debley3, Gurjit K. Khurana Hershey4, Hong Ji1,41Pyrosequencing Core, Cincinnati Children&#39;s Hospital, 2Division of Developmental Biology, Cincinnati Children&#39;s Hospital, 3Division of Pulmonary Medicine, Seattle Children&#39;s Hospital, 4Division of Asthma Research, Cincinnati Children&#39;s HospitalThis publication demonstrates methods for successful sampling and culture of nasal epithelial mucosa from children, and reprogramming these cells to induced Pluripotent Stem Cells (iPSCs).

march 2016 - this month in jove: rna interference in mosquitoes, ipscs derived from nasal epithelia, air sampling of atmospheric aerosols, and autologous micro-grafts for skin lesions

Here's a look at what's coming up in the <a href="http://www.jove.com/archive/109">March 2016 issue</a> of <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.
In <a href="http://www.jove.com/biology">JoVE Biology</a>, <a href="http://www.jove.com/science-education/5105/">RNA interference (RNAi)</a> is a natural mechanism of gene silencing that occurs via double-stranded RNA (dsRNA), which targets homologous DNA for degradation. This phenomenon allows researchers to selectively silence genes in many eukaryotes-making RNAi an extremely valuable tool for uncovering gene function. However, in the mosquito Anopheles gambiae, a major vector for malaria, RNAi has limited ability to target genes during developmental stages. This month, <a href="http://www.jove.com/video/53738">Regna et al.</a> present an RNAi protocol using direct injection during pupal development. After the pupae complete development, their adult phenotypes confirm the gene knockdown. This method expands the arsenal of genomic tools for vector insect research.
In <a href="http://www.jove.com/devbio">JoVE Developmental Biology</a>, induced pluripotent stem cells (iPSCs) have been generated from many cell types, and are valuable models for human development and disease. iPSCs are also valued for their potential applications in regenerative medicine. This month, <a href="http://www.jove.com/video/53814">Ulm et al.</a> demonstrate methods for sampling nasal epithelial mucosa from children, then culturing the samples to obtain nasal epithelial cells (NECs), which are reprogrammed into iPSCs. NECs are of particular interest because they're the primary cells infected with respiratory viruses, and are readily accessible during clinical visits. Therefore, this protocol facilitates patient-specific research in airway epithelial biology.
In <a href="http://www.jove.com/enviro">JoVE Environment</a>, filters are important tools in atmospheric aerosols research. These sampled filters collect ambient particles, such as endotoxins and biological aerosols, for analysis. In this issue, <a href="http://www.jove.com/video/53444">Lang-Yona et al.</a> use air-sampled filters for two complementary analyses of atmospheric biological particles: endotoxin and DNA. Specifically, they study endotoxin components of gram-negative bacterial cell walls, known collectively as lipopolysaccharide (LPS). In parallel, they perform a genomic evaluation of the sample's bacterial content. This method can produce highly accurate and reliable analyses for biological aerosol research.
In <a href="http://www.jove.com/med">JoVE Medicine</a>, researchers are finding new ways to treat wounds with a patient's own tissues, This month, <a href="http://www.jove.com/video/53579">Purpura et al.</a> describe a new method of creating autologous micrografts. When cultured on collagen sponges, these micro-grafts become bio-complexes ready to use in the treatment of skin lesions. These biocomplexes were applied in a patient, who showed good healing after 30 days. This new regenerative approach shows promise as an efficient, one-step treatment of acute and chronic lesions.
You've just had a sneak peek of the <a href="http://www.jove.com/archive/109">March 2016 issue</a> of <a href="http://www.jove.com/">JoVE</a>. Visit the website to see the full-length articles, plus many more, in <a href="http://www.jove.com/">JoVE: The Journal of Visualized Experiments</a>.

Watch this Scientific Journal Video about March 2016 - This Month in JoVE: RNA interference in Mosquitoes, iPSCs Derived from Nasal Epithelia, Air Sampling of Atmospheric Aerosols, and Autologous Micr

March 2016 - This Month in JoVE: RNA interference in Mosquitoes, iPSCs Derived from Nasal Epithelia, Air Sampling of Atmospheric Aerosols, and Autologous Micro-grafts for Skin Lesions

Here's a look at what's coming up in the March 2016 issue of JoVE: The Journal of Visualized Experiments.
In JoVE Biology, RNA interference (RNAi) is a ...

march-2016-this-month-jove-rna-interference-mosquitoes-ipscs-derived

Without Section

2012: A Year In Review

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE reached a major milestone by publishing our 1500th article. In this publication, in JoVE Bioengineering, Yu et al. described a method for analyzing the free radical composition of cigarette smoke. This method simulated cigarette puffing with a single-port smoking device, and combined it with electron spin resonance (ESR) spectroscopy to measure the degree to which antioxidant compounds can scavenge free radicals in cigarette smoke. A possible outcome of this research is the creation of less harmful cigarettes.
JoVE Bioengineering also contained the most-viewed article of 2012. Owczarczak et al. described a method for hacking a standard inkjet printer, loading ink cartridges with a cell-suspension "bioink," and printing lines of live cells onto glass slides. The thermal inkjet printing process creates temporary pores in the cell membrane, visualized by the incorporation of fluorescently labeled actin monomers. This concept, called "bioprinting," has many potential applications in cell and tissue engineering.
Also in 2012, JoVE Bioengineering featured a protocol by Hsia et al. for isolating and purifying spider silk protein, spinning it into fibers, and assessing the fiber strength. This protocol for laboratory-scale production of spider silk, which is stronger than tensile steel, can potentially be extended to large-scale manufacturing.
In JoVE Clinical and Translational Medicine, we featured a number of articles related to stroke, one of which. In one article, M&ouml;bius-Winkler et al. demonstrated placement of the WATCHMAN left atrial appendage occlusion device from Boston Scientific. This device is designed to prevent strokes by trapping clots within the left atrial appendage of the heart before they exit. Our authors demonstrated how to insert the guide wire through the femoral artery into the heart, advance the device into the left atrium, and deploy the device in the left atrial appendage.
Another article focused on stroke, Hamel et al. described the use of a driving simulator to study how patients compensate for visual field defects following stroke. By analyzing the compensatory gaze behavior of patients as they navigated through virtual driving courses with varying degrees of complexity, our authors see great potential for the use of driving simulators in stroke rehabilitation.
In JoVE Immunology and Infection, we published an article by Keyel et al. describing the real-time kinetics of immune cell responses to bacterial toxins using live cell microscopy. Combined with high-speed 3D confocal microscopy, this technique can also visualize the cellular repair response.
Also in JoVE Immunology and Infection, we featured an article by Yap et al. describing methods for diagnosing helminth infections in children. Helminths are parasitic worms that can infect the intestinal tract of humans and animals, and may be distinguished based on their morphology. The same article demonstrated how to measure the impact of helminth infections on physical fitness in children, which is a measure of overall health.
In JoVE Neuroscience, we featured a method by Hoffmann et al., who used tiny headphones to alter the auditory feedback in songbirds, and analyzed the computational and neurophysiological basis of vocal learning in birds when they adjust their singing in response to altered acoustic signals.
Also in JoVE Neuroscience, we moved from birds to bees with a method that assesses associative and non-associative tactile learning in honeybees. This method, by Mujagi&#263; et al., allowed bees to scan different metal surfaces with their antennae, then conditioned them to expect sugar water when their antennae touched particular surfaces, and analyzed the corresponding changes in antennal movement.
In 2012, we launched a brand-new section: JoVE Applied Physics, which features articles on subjects ranging from plasma physics to material science. The Applied Physics section also contained JoVE's 2000th video article, filmed in a synchrotron radiation facility, where Borisenko et al. showed how to determine the electronic structure of complex materials using angle-resolved photoemission spectroscopy.
In the JoVE General section, we published an article by Artioli et al. demonstrating the use of a portable gas analyzer to measure oxygen consumption during complex exercise - such as judo. By analyzing oxygen consumption and collecting blood for measuring plasma lactate concentration, this method can determine the relative contributions of different energy systems to specific aspects of complex exercise.
Finally, JoVE followed Dolhi et al. to a permanently ice-covered saline lake in Antarctica. There, scientists drilled through the ice to study single-celled microorganisms called protists that live in the harsh conditions of Antarctica.
This Year in Review was just a brief glimpse of a few of over 600 video articles that JoVE offered in 2012. Browse the JoVE archives to see thousands of other videos, and stay tuned for what's coming up this year in JoVE: The Journal of Visualized Experiments.

Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).

 
 
Here's a look at some of the milestones and highlights of the year 2012 in Journal of Visualized Experiments (JoVE).
At the start of 2012, JoVE ...