Induction of Adipogenesis in Isolated Endothelial Cells
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Results: CD31+CD34+ have Endothelial Phenotype and Display Depot- and Subject-specific Responses to Adipogenic Induction
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Conclusion
Transcription
This method can help answer key questions in the obesity field, such as identifying the key factors and mechanisms that prevent a robust adipogenic response during adipose tissue remodeling. The main advantage of this technique is that it allows i
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The differentiation of white and beige adipocytes from adipose tissue vascular progenitors bears potential for metabolic improvement in obesity. We describe protocols for a CD34+CD31+ endothelial cell isolation from human fat and for a subsequent in vitro expansion and differentiation into white and beige adipocytes. Several downstream applications are discussed.