Media Preparation, Splenocyte Isolation, and Challenge
3:02
RNA Isolation and cDNA Synthesis
4:50
Quantitative Polymerase Chain Reaction (qPCR)
5:40
Results: Clock Gene Expression
6:33
Conclusion
Transcription
This method can help answer key questions in the fields of immunology and chronobiology, such as how microbial components alter the molecular clock. The main advantages of this technique are that it is easy to perform, the effects of different mic
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This protocol describes a technique using mouse splenocytes to discover pathogen-associated molecular patterns that alter molecular clock gene expression.