Cochlear surface preparations allow visualization of the entire lens of the organ of Corti using immunohistochemistry and confocal imaging. It has been broadly used for investigation of specific cochlear pathologies of interest. We will modify the
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This article presents a modified cochlear surface preparation method that requires decalcification and use of a cell and tissue adhesive to adhere the pieces of cochlear epithelia to 10 mm round cover slips for immunohistochemistry in adult mouse cochleae.