S'identifier

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

1.3K Views

•

08:24 min

•

July 27th, 2021

DOI :

10.3791/61689-v

July 27th, 2021

•

1,310 Views

Jing Zhang*¹, Jing Huang*², Ishani Majumdar¹, Ryan C. James³, Julia Gichimu¹, Manikandan Paramasivam⁴, Durga Pokharel⁵, Himabindu Gali⁶, Marina A. Bellani¹, Michael M Seidman¹

¹Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, ²Institute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, ³Department of Molecular Biology and Genetics, Cornell University, ⁴Department of Cellular and Molecular Medicine, University of Copenhagen, ⁵Horizon Discovery, ⁶Boston University School of Medicine

Chapitres

Transcription

Résumé

Explorer plus de vidéos

Visualization

Replisome

Antigen tagged DNA Adduct

Immunodetection

Cell Cycle dependent Interactions

DNA Lesion

Dig TMP

UVA Light

Cytoskeleton Extraction

Formaldehyde Fixation

Methanol Fixation

Triton X 100 Permeabilization

Now Playing

08:24

Visualization of Replisome Encounters with an Antigen Tagged Blocking Lesion

1.3K Views

08:51

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Vidéos connexes

13.0K Views

07:11

Homemade Site Directed Mutagenesis of Whole Plasmids

Vidéos connexes

32.9K Views

07:44

High-throughput Purification of Affinity-tagged Recombinant Proteins

Vidéos connexes

14.0K Views

18:27

One-step Purification of Twin-Strep-tagged Proteins and Their Complexes on Strep-Tactin Resin Cross-linked With Bis(sulfosuccinimidyl) Suberate (BS3)

Vidéos connexes

18.5K Views

13:06

Visualization of miniSOG Tagged DNA Repair Proteins in Combination with Electron Spectroscopic Imaging (ESI)

Vidéos connexes

10.0K Views

08:51

Visualization of Surface-tethered Large DNA Molecules with a Fluorescent Protein DNA Binding Peptide

Vidéos connexes

10.6K Views

09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Vidéos connexes

8.7K Views

13:26

Visualization and Quantification of Mesenchymal Cell Adipogenic Differentiation Potential with a Lineage Specific Marker

Vidéos connexes

9.7K Views

08:16

Noninvasive Monitoring of Lesion Size in a Heterologous Mouse Model of Endometriosis

Vidéos connexes

8.0K Views