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Multiplexed Fluorescent Immunohistochemical Staining of Four Endometrial Immune Cell Types in Recurrent Miscarriage

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05:16 min

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August 4th, 2021

DOI :

10.3791/62931-v

August 4th, 2021

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3,111 Views

Yiwei Zhao¹, Gene Chi Wai Man¹^,², Loucia Kit Ying Chan¹, Xi Guo¹, Yingyu Liu³, Tao Zhang¹, Joseph Kwong¹^,⁴, Chi Chiu Wang¹^,⁵, Xiaoyan Chen³, Tin Chiu Li¹

¹Department of Obstetrics and Gynaecology, The Chinese University of Hong Kong, Prince of Wales Hospital, ²Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, Prince of Wales Hospital, ³Department of Obstetrics and Gynaecology, Shenzhen Baoan Women’s and Children’s Hospital, Shenzhen University, ⁴School of Medicine, Faculty of Medicine and Health Sciences, Keele University, ⁵Li Ka Shing Institute of Health Sciences; School of Biomedical Sciences, The Chinese University of Hong Kong

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Multiplexed Fluorescent Immunohistochemistry

Endometrial Immune Cells

Recurrent Miscarriage

Formalin fixed Paraffin embedded Tissue

Epitope Retrieval

Antibody Staining

Tyramide Signal Amplification

Microwave based Antigen Stripping

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